Circulating cell-free DNA in health and disease — the relationship to health behaviours, ageing phenotypes and metabolomics

Open access funding provided by Karolinska Institute. We also acknowledge the fnancial support provided by the European Commission through the FP7 large-scale integrating project “European Study to Establish Biomarkers of Human Ageing” (MARK-AGE; grant agreement No.: 200880). The Young Finns Study has been fnancially supported by the Academy of Finland: grants 322098, 286284, 134309 (Eye), 126925, 121584, 124282, 129378 (Salve), 117787 (Gendi), and 41071 (Skidi); the Social Insurance Institution of Finland; Competitive State Research Financing of the Expert Responsibility area of Kuopio, Tampere and Turku University Hospitals (grant X51001); Juho Vainio Foundation; Paavo Nurmi Foundation; Finnish Foundation for Cardiovascular Research; Finnish Cultural Foundation; The Sigrid Juselius Foundation; Tampere Tuberculosis Foundation; Emil Aaltonen Foundation; Yrjö Jahnsson Foundation; Signe and Ane Gyllenberg Foundation; Diabetes Research Foundation of Finnish Diabetes Association; This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreements: No 848146 for To Aition and grant agreement 755320 for TAXINOMISIS, European Research Council (grant 742927 for MULTIEPIGEN project), Tampere University Hospital Supporting Foundation and Finnish Society of Clinical Chemistry. The Health 2000 Survey was funded by the National Institute for Health and Welfare (THL), the Finnish Centre for Pensions (ETK), the Social Insurance Institution of Finland (KELA), the Local Government Pensions Institution (KEVA) and other organisations listed on the survey website (https:// thl.f/en/web/thl-biobank/for-researchers/sample-collections/ health-2000-and-2011-surveys). This analysis was supported fnancially by Yrjö Jahnsson Foundation (Grant 20197181) and Juho Vainio Foundation to L. Kananen, and by the Academy of Finland through its funding to the Centre of Excellence in Research of Ageing and Care (CoEAgeCare, grant numbers 326567 and 336670). M. Ala-Korpela was supported by a research grant from the Sigrid Juselius Foundation, Finland. J. Jylhävä was supported by the Swedish Research Council (2018–02077), the Loo & Hans Osterman Foundation and the Strategic Research Program in Epidemiology at Karolinska Institutet.

The data used in the current study cannot be stored in public repositories or otherwise made publicly available due to ethical restrictions. However, data are available upon request from the MARK-AGE, YFS, and Health 2000 survey for researchers who meet the criteria for access to confdential data. Data from the MARK-AGE study are available from the MARK-AGE steering committee (contact: Professor Alexander Bürkle, alexander.buerkle@uni-konstanz. de). Regarding YFS, investigators can submit an expression of interest to the chairman of the data sharing and publication committee (Professor Mika Kähönen, Tampere University, Finland). The Health 2000 data are available from THL on request, subject to the submission of approved study proposals and a data transfer agreement (contact: terveys-2000–2011@thl.f).